Objective To get an insight into the carriage status and epidemic characteristics of Bartonella in rodents in Zhejiang province for providing baseline data for the development of measures for the control and prevention of Bartonella infection. Methods DNA was extracted from the livers and spleens of mice and the beta-subunit-encoding gene (rpoB) of Bartonella was selected for PCR amplification, the positive products of which were sequenced and phylogeneticaly analyzed. Results One of 101 rodents captured in Panan county of Jinhua city in Zhejiang was positive for the rpoB gene of Bartonella with the isolation rate of Bartonella in Rattus tanezumi being 4.3％. Phylogenetic analysis showed that the isolated strain was of the same evolutionary branch as B. grahamii (GenBank accession No. AB426694.1) with a bootstrap value of 96%. Conclusion There exists Bartonella infection in rodents in Zhejiang province with R. tanezumi as its possible host. The isolated strain belongs to B. grahamii.

Objective To identify the prevalence of ticks infected with spotted fever group rickettsiae in mountain areas of Zhejiang province, and to explore the feasibility of the PCR approach using rickettsial outer membrane protein A (rOmpA) and citrate synthase (gltA) gene as the target genes. Methods The rOmpA and gltA gene specific fragments were assessed using the PCR method from 46 tick specimens in Tiantai Zuoxi county and Lin’an Xitianmu region. The positive samples were cloned and sequenced followed by cluster analysis. Results The rOmpA and gltA gene specific fragments of spotted fever group rickettsiae were detected from 2 Haemaphysalis longicornis of the 46 specimens, with similar nucleic acid sequences. However, the evolutionary positions of the spotted fever group rickettsia species were presumably different between the two. Conclusion The ticks infected with spotted fever group rickettsiae were found in some mountain areas of Zhejiang province. Spotted fever group rickettsiae were detected from the

【Abstract】 Objective To approach the feasibility of FlaB?PCR used for the detection of leptospira. Methods The proper  primer  was  synthesized  according  to  FlaB  gene and its sensitivity and specificity was evaluated compared to the primer G1/G2 recommended by public health industry standard. It was applied to leptospira detection of frog and rats which were from Longyou and Changshan. And it was identified by PCR. Results FlaB?PCR was highly sensitive and specific, and pathogenic leptospira DNA could be specifically amplified with the selected primer. The PCR results on leptospira isolated from Zhejiang province  in  2007 were in accordance with the serology results. The positive rate of the kidneys of rats and frogs was 20.94% by FlaB?PCR. Compared with the primer G1/G2 recommended by public health industry, the coincidence of two methods was 90.54%. Conclusion FlaB?PCR can detect the pathogenicity leptospira specifically and quickly, which can reflect the carrying rate of leptospira in wild animals accurately and efficiently and provide the evidence for leptospira control.

Objective To study active components of extract of Arisaema erubescens Schott against Oncomlania hupensis. Methods The snail mortality was calculated. Results The n-Butyl alcohol extract is the best active components against the snails. The mortality of snails is 76.67% and 100% after 100 mg/L n-Butyl alcohol extract treatment for 2 d and 3 d respectively. The molluscicidal activity of the plant tuber is higher than that of the plant overground part. After snails treated with 60 mg/L of n-Butyl alcohol extract from overground part and tuber for 2 d, the mortality of snails is 13.33% and 40.00% and treated for 4 d the mortality is 50.00% and 100% respectively. Conclusion The n-Butyl alcohol extract of tuber of A.erubescens may be the effective components against O.hupensis.

Objective To study the infection of Spotted fever Group Ricketfsiase in wild rodent and tick in Zhejiang province. Methods Polymerase chain reaction was used to detect Spotted fever Group Ricketfsiase DNA in tick and rodent's spleen. Results From Jindong district,Jinhua city,2 Rattus confucianus,10 Rattus lossea and 4 Apodemus agrarius were captured. All of them were negative by PCR. 8 specimens of the 49 Lxodes sinensis were positive.The positive rate was 16.33.Conclusion It showed preliminarily that the pathogen of Spotted fever Group Ricketfsiase occurred in Zhejiang,which should be further investigated.

Objective The rapid laboratory detection technology of Dengue fever was applied to provide important evidence in efficient diagnosis and epidemics control of Dengue. Methods Indirect immunofluorescent assay(IFA) was developed to detect the sera from suspicious Dengue patients in 2003-2004. Results By the IFA assay,three Dengue fever epidemics was confirmed including one outbreak. Conclusion The imported Dengue epidemics occurred in Zhejiang in 2003-2004.